3^rd European Food Safety and Standards Conference

Biography

Biography: Pavla Martinkova

Abstract

A peroxidase biosensor is highly demanded in food, healthcare, pharmaceutical and environmental analysis. H2O2 is often a residue component of non-food product. Moreover, H2O2 is used as an antibacterial agent added to milk or it is used for sterilization of equipment related to food and beverage during the both technological manufacturing and packaging. In the European Union countries, contain of H2O2 higher than 0.1% of is not allowed in these products. If the level of H2O2 exceeds 6 %, health damage or at least skin irritation can be expected. Due to toxicity of H2O2 in higher concentrations sensitive method for its detection is required. In this work, a voltammetric biosensor based on horseradish peroxidase, magnetic particles and Prussian blue entrapped in chitosan membrane onto carbon working electrode was proposed as a suitable tool for the assay purposes. Magnetic particles having pseudo-peroxidase activity catalyzed reduction of H2O2 and Prussian blue enabled highly sensitive detection at low potential. Square wave voltammetry was used as detection method. Concentration curve replies Michaelis equation with correlation coefficient 0.999 and limit of detection was set to be 0.03 % of H2O2. Uric acid, ascorbic acid, trolox, acetaminophen and reduced glutathione were measured as interferents and no significant influence on measured method was observed when presented in equivalent concentrations like the H2O2. No effect of matrix (tooth paste, hand cream and skin tonic) assay was detected. On the basis of gained results, method was considered as highly sensitive, accurate and fast assay for detection of H2O2.