3^rd European Food Safety and Standards Conference

Biography

Biography: Gamal M. Hamad1

Abstract

Aflatoxins found in dairy products and animal feeds represent a serious problem for human and animal health. In present study, combined effect of probiotic bacteria; Lactobacillus acidophilus, Bifidobacterium bifidum) and yeast strains; kluyveromyces lactis and Saccharomyce cerevisiae) were used for detoxification of aflatoxins (B1, B2, G1, G2) in animals fed on flour contains some of these toxins. Aflatoxins’ residues were determined in the mothers and their infants using HPLC. The in vitro study revealed that the probiotic bacterium lactobacillus, B. bifidum, and their combination were able to lower the level of aflatoxins in PBS solutions by 30%, 44%, and 59%; respectively. Similarly, each of the two yeast strains, S. cerevisiae and K. lactus, showed efficient detoxification of aflatoxins by percentage 50%, with a higher efficiency when used in combination up to 75%. Interestingly, higher rate of detoxification (94%) was achieved after 72 hr treatment, by using combination of the two bacterial and the two yeast strains. Therefore, we select these combinations, mixed altogether with flour containing the four aflatoxin, and then the toxins’ residues were determined in the flour after 72 hours. Results revealed that aflatoxins’ levels were reduced by 87% compared to non-treated flour. We applied the previous trial on the animals and aflatoxins were determined in the serum of the adult rat and their infants. Group 1 and group 2 serum did not contain any type of the four afaltoxins. Whenever, group three serum adult rat 1 contains aflatoxin (G2,G1,B2,B1) with perecentage 12.5%, infants 1 serum contains both afaltoxins (M2, M1) with percentage 10% but the infants 2 and 3 serums contains afaltoxins (M2, M1). Moreover, Group 4 contains adult 1 and its serum contains aflatoxins (G1, B2, B1), and infants 1,2 and 3 contains (M2, M1). Creatinin in the adult rat treated with afaltoxins was 85 but in infants it was around 74. On other hand, creatinin in the serum of control animals was ranged from 41 to 44. But in the adult rat treated with afaltoxin-probiotic mix, creatinin was about 46 and it ranged btween 31 to 34 in the infants. Urea in control animals (adult rat and their infants were ranged from 37 to 49. But in the treated with aflatoxin only adult rat and their infants it was ranged from 78 to 91. In case of mothers treated with aflatoxin and probiotic bacteria; urea level in the serum of adult rat and their babies ranged between 42 to 50. Liver GOT enzyme in the control animals (adult rat and their infants) were ranged between 9.19. Whenever, adult rat treated with aflatoxin only and their infants were ranged between 31 to 73. This reading was decreased in animals