Miroslav Pohanka
University of Defense, Czech Republic
Title: Construction of electrochemical acetylcholinesterase based biosensor with nanostructured membrane
Biography
Biography: Miroslav Pohanka
Abstract
Acetylcholinesterae (AChE) based biosensors are analytical tools suitable for a fast and simple assay of neurotoxic compounds. In the present study, we focused our activity at construction of biosensors consisting of a screen printed sensor having on its surface immobilized AChE. Several immobilization protocols including immobilization onto spherical graphite particles and embedding into sol-gel were selected for construction of a voltammetric biosensor. The determination of AChE activity was based on electrochemical oxidation of thiocholine originating from an enzymatic substrate acetylthiocholine. The constructed biosensor was calibrated using the toxic pesticide carbofuran respective organophosphate diisopropylfluorphosphate resulting in limit of detection around 50 nmol/l. The biosensor seems to be suitable for practical performance in assay of pesticides in food and beverages, agrochemistry and similar fields.