Lydia Ninan Lestario
Satya Wacana Christian University
Indonesia
Title: Extraction and identification of anthocyanin of Java Prune (Kopsia pruniformis) fruit peels
Biography
Biography: Lydia Ninan Lestario
Abstract
Java Prune (Kopsia pruniformis) is a tropical plant which has dark purple color on the fruit peelindicated high anthocyanin content that had potency as source of safe natural colorant.The aims of this research were to determine the total anthocyanin content of Java Prune (Kopsia pruniformis), to compare several solvents that could extract highest anthocyanin and to identify kind of its anthocyanin.The total anthocyanin content was determined by pH differential method whereas the identification of anthocyanidin was based on the Rf value, maximum absorbance of the spots and time retention on HPLC with anthocyanidin standard. The results showed that the total anthocyanin content ofjava prune fruit peels was 3602.13±43.15 mg/100g (based on fresh weight), the best solvent for extraction of anthocyanin was 1% tartaric acid which could extract 56.02% anthocyanin (about 2017.89±86.95 mg/100 gr). It was found 4 kinds of anthocyanins in java prune fruit peels: Cyanidin-3-glucoside, Petunidin-3-glucoside, Pelargonidin-5-glucoside and Pelargonidin-3-rhamnoside in which the major was Cyanidin-3-glucoside.