Food Safety and Regulatory Measures

Biography

Biography: G. Enan

Abstract

One hundred and fifty Staphylococcus aureus (S. aureus) isolates were obtained from clinical samples, characterized and identified. Out of this collection, thirty isolates were found to be methicillin resistant Staphylococcus aureus (MRSA). Only one strain (isolate No.P59) out of this subgroup exhibited reduced susceptibility to vancomycin, recoding 8 and 10 µg/mL MIC (minimum inhibitory concentration) and MBC (minimum bactericidal concentration), respectively. This strain was concluded as vancomycin intermediate S. aureus (VISA) and designated VISA P59. This VISA P59 further identified by 16S r RNA sequence analysis and PCR analysis revealed that it contained methicillin resistant gene (mec A) in its genome but did not contain vancomycin resistant genes (van A and van B) .The basic subunits of glycinin isolated from soybean protein (GBS) inhibited vigorously VISA P59 as qualitatively visualized by agar well diffusion method. GBS antimicrobial activity against VISA was stable over a wide pH range and under different incubation temperatures. It could prevent the in vitro growth of VISA P59 at 37 ºC for 4 days and inhibited almost 99% of viable growth of VISA P59 in minced beef meat stored at 37 ºC for 14 days. No animal toxicity was observed with GBS.